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KMID : 0368420040470030187
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Journal of Plant Biology
2004 Volume.47 No. 3 p.187 ~ p.193
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Detection of insect Juvenile hormone III and Its precursors fromin Vitro plantlets ofCyperus aromaticus
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Weng Chan Chee
Keng Chan Lai
Lim Boey Peng
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Abstract
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Juvenile hormone III (JH III) is one of the five closely related sesquiterpenoid compounds important in the regulation of an insect physiological processes in both the developing and the mature insect. JH III and its biosynthetic intermediates, farnesol and methyl farnesoate were detected in thein vitro Cyperus aromaticus plantlets and the mature plants growing in the field. A higher amount of methyl farnesoate (52.7%) and farnesol (31.4%) were found to be present in thein vitro plantlets as compared to the field grown mother plants which contained lower methyl farnesoate (19.5%) and farnesol (14.8%). On the other hand, the mature mother plant (4 months old) contained a higher percentage of JH III than thein vitro plantlets. The estimated content of JHIII analyzed by gas chromatography indicated that the amount of JHIII in the 10 weeks oldin vitro plantlets and the four months old mature plants of C.aromaticus was 0.076 μg/g and 0.085 μg/g respectively. This study indicated thatin vitro culture system could be a potential tool for the production of this natural derived bio-insecticide and could provide a material source for further investigations of the biosynthesis of JH III inC. aromaticus.
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KEYWORD
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Cyperus aromaticus, micropropagation, gas chromatography-mass spectroscopy, insect juvenile hormone III, farnesol, methyl farnesoate
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